Methods of isolation and identification of Enterobacteriaceae

Abstract

Aim and objectives: The purpose of this study on the isolation and identification of Enterobacteriaceae is aimed at bacteriological examination, following the basic examination of the microbiological diagnosis of pathological samples, being represented by the cultivation of feces on specific culture media. Methods: We conducted a prospective and retrospective study on eighty-six isolated clinical trials, including Enterobacteriaceae, to identify and highlight genotypic and/or phenotypic characteristics between strains isolated from single infections and those isolated from recurrent cases based on microbiological diagnosis registered in the bacteriological register of the medical analysis laboratory, SC Diaser, Oradea. Results: Antibodies detected by the Western blot method are guided against the 3 species of Yersinia: enterocolitica, pseudotuberculosis and pestis. For the detection of specific anti-Yersinia antibodies, the antigen-loaded band is incubated together with the patient's diluted serum. The weakly selective media, MacConkey, Eosin-Methylene Blue, allow the growth of all Enterobacteriaceae lactase positive and negative, even other groups of gram-positive bacilli. Conclusions: Poorly selective media, MacConkey, Eosin methylene blue, allow the growth of all Enterobacteriaceae lactase positive and negative, even other groups of gram-positive bacilli.