Diagnosis of laboratory in the infections produced by Clostridium Difficile

Abstract

Aim and objectives: Regarding the diagnosis of laboratory in the infections produced by the gram-positive bacillus, anaerobe, Clostridium Difficile has as target the bacteriological examination, following the basic examination of the microbiological diagnosis of the infectious diarrheic syndrome, being represented by the cultivation of the fecal matters on specific culture mediums. Material and methods: We accomplished a prospective and retrospective study, on eighty of clinical isolated samples of C. Difficile to identify and underline the genotypic and/or phenotypic characteristics between the isolated strains from cases of unique infections and those isolated from recurrent cases, based on the microbiologic diagnosis registered in the bacteriological register of the laboratory of medical analysis, S.C. Diaser, Oradea. Results and discussions: The most important factor of virulence is the release of multiple toxins, and namely large glycosylated exotoxins A and B. Conclusions: The placing of the diagnosis of laboratory in regard to Clostridium Difficile, is based on the culture and the detection of toxins in fecal samples.