Evaluation of the cytotoxic and anti-angiogenic potential of flavone apigenin using the B164A5 mouse melanoma cell line

Abstract

Aim and objectives: The present study was designed in order to evaluate the cytotoxic and anti-angiogenic potential of apigenin using the B164A5 mouse melanoma cell line. Material and methods: The effect of apigenin against B164A5 mouse melanoma cell line was evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and Lactate dehydrogenase (LDH) assays. For the determination of the anti-angiogenic potential CAM assay was performed. Results: At 72h post-stimulation, Api provoked a dose-dependent decrease of melanoma cells viability. Significant statistic results were found at the concentrations of 10 µM 30µM and 60 µM for reduction of proliferation, respectively 30µM and 60 µM for cytotoxicity. Data from this study showed that at both tested doses Api reduced the migration potential of melanoma cells. Conclusions: Apigenin induced, dose-dependent antiproliferative, cytotoxic and anti-angiogenic effects on B164A5 cells. The compound represents a promising candidate for further studies of experimental animal models of melanoma.