Screening Periodontitis Associated with Diabetes by Using Oxidative Stress Biomarkers in Blood and Gingival Tissue

Abstract

Introduction: Periodontitis and diabetes have a two-way relationship but the mechanisms implicated in their association are still unclear. Aim and scope: We aimed to evaluate local and circulatory oxidative stress in an experimentally induced periodontitis associated with diabetes on Wistar rats. Materials and methods: Forty Wistar rats were divided in: control group (C) and periodontitis (P), diabetes (D) and periodontitis associated with diabetes (DP) group. Blood and gingival malondialdehyde (MDA) and blood glutathione (GSH), oxidized glutathione (GSSG), GSH/GSSG ratio, and catalase (CAT), and gingival CAT were determined. Results: blood and gingival MDA increased significantly in P and DP and blood GSH, GSSG, GSH/GSSG, CAT and gingival CAT decreased significantly. Circulatory MDA, GSH, GSSG and GSH/GSSG were significantly modified in DP compared to P. Conclusion: oxidative stress is implicated in periodontitis and periodontitis associated with diabetes and circulatory MDA, GSH, GSSG and GSH/GSSG could indicate a risk of their association.